Multi-Leu peptide

Multi-Leu peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Ac-LLLLRVKR-NH2

Ac-Leu-Leu-Leu-Leu-Arg-Val-Lys-Arg-NH2

8

95.1%

C₄₉H₉₄N₁₆O₉

1051.37

Synthetic

Powder

Multi-Leu peptide is a peptide identified from a partial combinatorial library that is selective for the proprotein convertase PACE4, notably being 20 fold selective over furin, a closely related enzyme with similar structural characteristics. Proprotein convertases play an important role in protein precursor activation through processing at paired basic residues and PACE4 specifically has an important role in prostate cancer. Consistent with inhibition of PACE4, Multi-Leu peptide significantly reduces the proliferation of DU145 and LNCaP prostate cancer derived cell lines and induces G0/G1 cell cycle arrest.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Levesque et al (2012) The Multi-Leu peptide inhibitor discriminates between PACE4 and furin and exhibits antiproliferative effects on prostate cancer cells. J Med Chem. 55(23) 10501 PMID: 23126600
• Kwiatkowska et al (2019) Enhanced anti-tumor activity of the Multi-Leu peptide PACE4 inhibitor transformed into an albumin-bound tumor-targeting prodrug. Sci Rep. 9(1) 2118 PMID: 30765725

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.