MLCK inhibitor peptide 18

MLCK inhibitor peptide 18

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-RKKYKYRRK-NH2

H-Arg-Lys-Lys-Tyr-Lys-Tyr-Arg-Arg-Lys-NH2

9

95.44%

C₆₀H₁₀₅N₂₃O₁₁

1324.62

Synthetic

Powder

MLCK inhibitor peptide 18 is a selective cell permeable inhibitor of myosin light chain kinase with an IC50 of 50 nM. MLCK inhibitor peptide 18 displays 4000-fold selectivity over CaM kinase II and does not inhibit PKA. In vitro and in vivo treatment with the MLCK inhibitor peptide 18 attenuates the increased epithelial monolayer permeability and occludin endocytosis caused by intestinal anoxia/reoxygenation (A/R) injury.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Lukas et al (1999) Identification of novel classes of protein kinase inhibitors using combinatorial peptide chemistry based on functional genomics knowledge. J.Med.Chem. 42 910 PMID: 10072688
• Jin and Blikslager (2016) Myosin light chain kinase mediates intestinal barrier dysfunction via occludin endocytosis during anoxia/reoxygenation injury. American J. Physiology-Cell Physiology 311(6) C996 PMID: 27760753
• Huang et al (2020) Wogonoside alleviates colitis by improving intestinal epithelial barrier function via the MLCK/pMLC2 pathway. Phytomedicine 68 153179 PMID: 32062328

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.