Recombinant MGMT protein (His tag)

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This recombinant protein product features a stable and soluble fragment (residues 1-207) of the human Methylated-DNA--protein-cysteine methyltransferase (MGMT), also known as O-6-methylguanine-DNA-alkyltransferase. This fragment is optimized for structural and biochemical studies, offering researchers a functional unit to investigate the enzyme's critical role in DNA repair mechanisms.

The full-length MGMT protein, encoded by the MGMT gene in Homo sapiens, plays a vital role in cellular defense by repairing alkylation damage in DNA. Specifically, it addresses the mutagenic and cytotoxic effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) lesions through a stoichiometric transfer of the methyl group to a cysteine residue within the enzyme. This irreversible reaction inactivates MGMT but effectively restores DNA integrity, highlighting its importance in genome maintenance. MGMT is localized to the nucleus, where it directly interacts with DNA to execute its repair function, making it a key subject in cancer research and therapeutic development.