MeO-Suc-Arg-Pro-Tyr-pNA peptide

MeO-Suc-Arg-Pro-Tyr-pNA peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

MeO-Suc-RPY-pNA

MeO-Suc-Arg-Pro-Tyr-pNA

3

95.47%

C₃₁H₄₀N₈O₉

668.70

82564-18-9

25108799

Synthetic

Powder

MeO-Suc-RPY-pNA is a colorimetric substrate for serine proteases, including trypsin, chymotrypsin, kallikrein-related peptidase 7 (KLK7), and kallikrein-related peptidase 3/prostate-specific antigen. Serine proteases preferentially bind to and cleave the Arg-Pro-Tyr (RPY) peptide sequence to release p-nitroanilide (pNA), which can be quantified by colorimetric detection at 405 nm as a measure of serine protease activity.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Pejler G, Sadler JE. Mechanism by which heparin proteoglycan modulates mast cell chymase activity. Biochemistry. 1999 Sep 14;38(37):12187-95. doi: 10.1021/bi991046b. PMID: 10508424.
• de Veer SJ, Furio L, Swedberg JE, Munro CA, Brattsand M, Clements JA, Hovnanian A, Harris JM. Selective Substrates and Inhibitors for Kallikrein-Related Peptidase 7 (KLK7) Shed Light on KLK Proteolytic Activity in the Stratum Corneum. J Invest Dermatol. 2017 Feb;137(2):430-439. doi: 10.1016/j.jid.2016.09.017. Epub 2016 Sep 30. PMID: 27697464.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.