MART 1 26-35 mutant (HLA-A*02:01) 27L peptide

MART 1 26-35 mutant (HLA-A*02:01) 27L peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-ELAGIGILTV-OH

H-Glu-Leu-Ala-Gly-Ile-Gly-Ile-Leu-Thr-Val-OH

10

95.82%

C₄₅H₈₀N₁₀O₁₄

985.17

Synthetic

Powder

Antigen Peptide [Leu27] - Melan - A MART-1 26-35 (HLA-A*02:01) ELAGIGILTV for stimulation of antigen-specific T cells in T cell assays such as ELISPOT, ICS, cytotoxity or proliferation assays. This Melan-A (26-35) analog, Leu substituted for Ala at position 27, shows better HLA-A*0201 binding properties as well as better immunogenicity and antigenicity than the natural Melan-A (26-35)

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Ekeruche-Makinde J, Clement M, Cole DK, Edwards ES, Ladell K, Miles JJ, Matthews KK, Fuller A, Lloyd KA, Madura F, Dolton GM, Pentier J, Lissina A, Gostick E, Baxter TK, Baker BM, Rizkallah PJ, Price DA, Wooldridge L, Sewell AK. T-cell receptor-optimized peptide skewing of the T-cell repertoire can enhance antigen targeting. J Biol Chem. 2012 Oct 26;287(44):37269-81. doi: 10.1074/jbc.M112.386409. Epub 2012 Sep 5. PMID: 22952231; PMCID: PMC3481325.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.