MAGE-C1 (1083-1091)/p1083 peptide

MAGE-C1 (1083-1091)/p1083 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-KVVEFLAML-OH

H-Lys-Val-Val-Glu-Phe-Leu-Ala-Met-Leu-OH

9

95.02%

C₅₀H₈₄N₁₀O₁₂S

1049.32

Synthetic

Powder

MAGE-C1 (CT-7) is a cancer-testis antigen that is expressed by tumor cells in >70% of myeloma patients and elicits a humoral response in up to 93% of patients with CT-7+ myeloma. MAGE-C1 (1083-1091)/p1083 peptide(KVVEFLAML) is the first CD8+ T-cell epitope of CT-7 to be reported and may be useful targets for T-cell therapy or vaccination of myeloma patients. MAGE-C1 (1083-1091)/p1083 is a HLA-A2-restricted CT-7 peptide.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Anderson LD Jr, Cook DR, Yamamoto TN, Berger C, Maloney DG, Riddell SR. Identification of MAGE-C1 (CT-7) epitopes for T-cell therapy of multiple myeloma. Cancer Immunol Immunother. 2011 Jul;60(7):985-97. doi: 10.1007/s00262-011-1009-3. Epub 2011 Apr 3. PMID: 21461886; PMCID: PMC3183483.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.