LTX-315 peptide

LTX-315 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

K-K-W-W-K-K-W-Dip-K-NH2

Lys-Lys-Trp-Trp-Lys-Lys-Trp-Dip-Lys-NH2

9

95%

C₇₈H₁₀₆N₁₈O₉

1439.79

Synthetic

Powder

LTX-315 (Synonyms: K-K-W-W-K-K-W-Dip-K-NH2) is a synthetic anticancer peptide, has the potential to adopt an amphipathic helical coil structure and was designed for intralesional treatment of tumors. LTX-315 was highly active against both murine and human melanoma cell lines in vitro, while displaying low EC50 cytotoxic activity against human red blood cells. Furthermore, when syngeneic B16 melanomas were treated intralesionally, a majority of the animals (~80%) treated with LTX-315 experienced a complete and long-lasting tumor regression.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Camilio KA, Rekdal O, Sveinbju00f6rnsson B. LTX-315 (Oncoporeu2122): A short synthetic anticancer peptide and novel immunotherapeutic agent. Oncoimmunology. 2014

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.