LP17 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

LP17 is a synthetic peptide derived from the complementary determining region-3 and the "F" β-strand of the extracellular domain of TREM-1. It functions as an inhibitory peptide by competitively binding to TREM-1, thereby blocking its interaction with natural ligands. LP17 has been shown to effectively suppress TREM-1-mediated signaling pathways, including the activation of SYK and downstream pro-inflammatory cascades such as CARD9/NF-κB and NLRP3/caspase-1. This peptide also attenuates microglial M1 polarization and reduces the production of inflammatory cytokines and chemokines.

In experimental models, LP17 demonstrates the ability to mitigate neuroinflammatory responses by inhibiting TREM-1/SYK interactions. This leads to decreased levels of inflammatory mediators such as IL-1β, IL-18, and IL-6, as well as reduced chemokine expression. Additionally, LP17 has been observed to diminish microglial pyroptosis by lowering the levels of GSDMD and its N-terminal fragment, which are critical for the formation of membrane pores and the release of intracellular inflammatory factors. The peptide's mechanism highlights its potential role in modulating innate immune responses in the central nervous system.

• Xu P, Zhang X, Liu Q, Xie Y, Shi X, Chen J, Li Y, Guo H, Sun R, Hong Y, Liu X, Xu G. Microglial TREM-1 receptor mediates neuroinflammatory injury via interaction with SYK in experimental ischemic stroke. Cell Death Dis. 2019 Jul 19;10(8):555. doi: 10.1038/s41419-019-1777-9. PMID: 31324751; PMCID: PMC6642102.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.