Liraglutide peptide

Liraglutide peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

HAEGTFTSDVSSYLEGQAAK(gamma-Glu-palmitoyl)EFIAWLVRGRG

His-Ala-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Val-Ser-Ser-Tyr-Leu-Glu-Gly-Gln-Ala-Ala-Lys(palm-gama-Glu)-Glu-Phe-Ile-Ala-Trp-Leu-Val-Arg-Gly-Arg-Gly

31

98.09%

C₁₇₂H₂₆₅N₄₃O₅₁

3751.2

Synthetic

Powder

Liraglutide, the second GLP-1 analog approved in 2009 in Europe and 2010 in USA under the trade name Victoza (Novo Nordisk). Liraglutide is a synthetic molecule produced using DNA recombinant technology. Compared to native GLP-1, it has an addition of a 16 carbon fatty-acid side-chain at Lys26 and an Arg34Lys substitution (Neumiller et al., 2010). After s.c. injection, only 1–2% of liraglutide circulates as free peptide in the plasma as the rest is noncovalently bound to albumin as ensured by the fatty acid side-chain (Zhang et al., 2012). This prolongs the half-life of liraglutide to 11–15 h approximately and hence administration once daily is needed.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Malm-Erjefalt M, Bjornsdottir I, Vanggaard J, Helleberg H, Larsen U, Oosterhuis B, van Lier JJ, Zdravkovic M, Olsen AK: Metabolism and excretion of the once-daily human glucagon-like peptide-1 analog liraglutide in healthy male subjects and its in vitro degradation by dipeptidyl peptidase IV and neutral endopeptidase. Drug Metab Dispos. 2010 Nov;38(11):1944-53. doi: 10.1124/dmd.110.034066. Epub 2010 Aug 13.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.