LFRFamide neuropeptide

LFRFamide neuropeptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GNLFRF-NH2

H-Gly-Asn-Leu-Phe-Arg-Phe-NH2

6

95.95%

C₃₆H₅₃N₁₁O₇

751.87

Synthetic

Powder

LFRFamide neuropeptide is a neuropeptide that has been characterized in the common cuttlefish (cephalopod species Sepia officinalis). It is a member of the LFRFamide subfamily, which was recently described in gastropod mollusks.
LFRFamide neuropeptide is synthesized using standard peptide synthesis procedures, including in situ activation with HBTUHOBt and DIEA, and protection of reactive side-chains with Pbf sulfonylamide and Trt amide. The peptide is purified by reversed-phase HPLC and its purity is higher than 99.9%.
LFRFamide neuropeptide has been shown to induce an increase in frequency, tonus, and amplitude when applied to a perfused rectum, but it does not have any effect on the contractile activity of the oviduct or other reproductive organs in the cuttlefish species.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Zatylny-gaudin C, Bernay B, Zanuttini B, Leprince J, Vaudry H, Henry J. Characterization of a novel LFRFamide neuropeptide in the cephalopod Sepia officinalis. Peptides. 2010;31(2):207-14.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.