KYL peptide

KYL peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-KYLPYWPVLSSL-OH

H-Lys-Tyr-Leu-Pro-Tyr-Trp-Pro-Val-Leu-Ser-Ser-Leu-OH

12

95.02%

C₇₄H₁₀₈N₁₄O₁₇

1465.73

Synthetic

Powder

KYL is an EphA4 receptor tyrosine kinase inhibitor with a Kd of 0.8 μM. KYL inhibits EphA4-EphrinA5 interactions with an IC50 of 6.34 μM. Through EphA4 inhibition, KYL can prevent dendritic spine reduction, the blocking of LTP induction and neuronal apoptosis caused by amyloid-β oligomers (AβOs). EphA4-/- neurons or sh-EphA4-transfected neurons showed reduced synaptotoxicity by AβOs. EphA4/c-Abl activation is a key-signalling event that mediates the synaptic damage induced by AβOs.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Lamberto et al (2012) Distinctive binding of three antagonistic peptides to the ephrin-binding pocket of the EphA4 receptor. Biochem. J. 445 47 PMID: 22489865
• Vargas et al (2014) EphA4 activation of c-Abl mediates synaptic loss and LTP blockade caused by amyloid-β oligomers. PLoS One 9 e92309 PMID: 24658113
• Tamura et al (2020) EphA4 regulates Aβ production via BACE1 expression in neurons. FASEB J. 34(12):16383 PMID: 33090569

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.