KW- peptide

KW- peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-EEFKWKFKEE-OH

H-Glu-Glu-Phe-Lys-Trp-Lys-Phe-Lys-Glu-Glu-OH

10

95.12%

C₆₇H₉₄N₁₄O₁₉

1399.54

Synthetic

Powder

A robust two-component hydrogel from complementary decapeptides, KW+/p2 (KKFEWEFEKK) and KW-/p1 (EEFKWKFKEE). The design of these complementary peptides included the simple ‘hphphp’ repeating pattern (h = hydrophobic residue, p = polar residue) that is known to favour β-sheet structures, with tryptophan included to enhance aromatic pairing interactions and to allow peptide concentrations to be accurately determined. Hydrogels are produced by the self-assembly of peptides p1 + p2 into antiparallel β-sheets, which undergo hierarchical self-assembly into different fibrillar structures including large twisted fibres.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• King PJ, Giovanna Lizio M, Booth A, Collins RF, Gough JE, Miller AF, Webb SJ. A modular self-assembly approach to functionalised β-sheet peptide hydrogel biomaterials. Soft Matter. 2016 Feb 14;12(6):1915-23. doi: 10.1039/c5sm02039e. Epub 2016 Jan 6. PMID: 26702608.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.