KSLW peptide

KSLW peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

H-KKVVFWVKFK-NH2

H-Lys-Lys-Val-Val-Phe-Trp-Val-Lys-Phe-Lys-NH2

10

95.8%

C₆₈H₁₀₆N₁₆O₁₀

1307.67

Synthetic

Powder

KSLW, a synthetic antimicrobial peptide, was demonstrated to be chemotactic for neutrophils in micromolar amounts and has a broad spectrum of antimicrobial activity against Gram (+) and Gram (-) bacteria, fungi, and viruses. It is a decapeptide composed of the amino acid sequence KKVVFWVKFK-NH2. KSLW enhances F-actin polymerization in neutrophils and can inhibit oxygen radical production in PMA- and LPS-stimulated neutrophils, and thus to enhance neutrophil chemotaxis and function.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Williams RL, Sroussi HY, Leung K, Marucha PT. Antimicrobial decapeptide KSL-W enhances neutrophil chemotaxis and function. Peptides. 2012 Jan;33(1):1-8. doi: 10.1016/j.peptides.2011.11.022. Epub 2011 Dec 16. PMID: 22197491.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.