KP1 peptide

KP1 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-FQGTFPDGFLWAVGSAAYQTEGGWQQHGKG-OH

H-Phe-Gln-Gly-Thr-Phe-Pro-Asp-Gly-Phe-Leu-Trp-Ala-Val-Gly-Ser-Ala-Ala-Tyr-Gln-Thr-Glu-Gly-Gly-Trp-Gln-Gln-His-Gly-Lys-Gly-OH

30

95.63%

C₁₄₉H₂₀₃N₃₉O₄₃

3228.42

Synthetic

Powder

KP1 is a peptide representing the sequence Phe57 to Lys86 of the anti aging protein Klotho, and is one of the buried β-strands in the N-terminal domain. KP1 mimics the anti-fibrotic action of Klotho by constraining TGF-β signaling, KP1 acts through binding to TGF-β receptor 2 (TβR2) and disrupting TGF-β/TβR2 interaction. KP1 inhibits multiple downstream TGF-β pathways including activation of Smad2/3 and mitogen activated protein kinases. In mouse models of renal fibrosis, KP1 preserves kidney function, ameliorates renal fibrosis and restores endogenous Klotho expression.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Yuan (2022) A Klotho-derived peptide protects against kidney fibrosis by targeting TGF-β signaling. Nat Commun. 13(1) 438 PMID: 35064106

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.