KLPGWSG peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

• KLPGWSG polypeptide is a novel bioactive peptide with significant potential in neural tissue engineering. It was identified through Phage Display technique on murine neural stem cells (NSC) and was found to be present in proteins involved in stem cell maintenance and differentiation.
• This peptide binds to molecules on the cell surface of NSC and has been shown to enhance neuronal differentiation of NSC when added to the differentiation medium or linked to a self-assembling peptide (SAP) scaffold. When conjugated to a FITC fluorochrome, it can potentially be used as an NSC marker. The peptide does not substitute for or increase the proliferative effect of epidermal growth factor (EGF) on NSC and does not prevent cell death in the absence of EGF and basic fibroblast growth factor (bFGF). However, it positively modulates the neuronal differentiation of NSC, making it a promising candidate for biomimetic studies and stem cell therapies aimed at nervous system regeneration.
• The KLPGWSG-functionalized SAP scaffolds, such as Ac-KLP, with appropriate stiffness, support cell attachment and survival, and show enhanced neuronal differentiation compared to non-functionalized scaffolds, suggesting a synergistic effect of the peptide's presence and scaffold properties.

• Caprini A, Silva D, Zanoni I, Cunha C, Volontè C, Vescovi A, Gelain F. A novel bioactive peptide: assessing its activity over murine neural stem cells and its potential for neural tissue engineering. N Biotechnol. 2013 Jun 25;30(5):552-62. doi: 10.1016/j.nbt.2013.03.005. Epub 2013 Mar 26. PMID: 23541699.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.