JCpep7 (plants) peptide

JCpep7 (plants) peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

KVFLGLK

Lys-Val-Phe-Leu-Gly-Leu-Lys

7

98.67%

804.03

Synthetic

Powder

​A novel cell membrane affinity chromatography method screened Jatropha curcas, isolating the cationic antimicrobial peptide JCpep7 (KVFLGLK).​​ JCpep7 exhibited broad activity against Gram-negative and Gram-positive bacteria (including Salmonella, Shigella, Pseudomonas, Staphylococcus, Bacillus, and Streptococcus), with MICs between 24 and 64 µg/mL. FTIR and TEM studies revealed its mechanism: disrupting cell walls and membranes, leading to lysis. This demonstrates membrane affinity chromatography's potential for high-throughput AMP discovery from J. curcas.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Xiao J, Zhang H, Niu L, Wang X. Efficient screening of a novel antimicrobial peptide from Jatropha curcas by cell membrane affinity chromatography. J Agric Food Chem. 2011 Feb 23;59(4):1145-51. doi: 10.1021/jf103876b. Epub 2011 Jan 26. PMID: 21268582.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.