iRGD peptide

iRGD peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

c(CRGDKGPDC) (Disulfide bridge:Cys1-Cys9)

H-Cys-Arg-Gly-Asp-Lys-Gly-Pro-Asp-Cys-OH (Disulfide bridge:Cys1-Cys9)

9

98.28%

C₃₅H₅₉N₁₃O₁₄S₂

948.03

Synthetic

Powder

The peptide CRGDKGPDC is a tumor-penetrating peptide that is used to deliver drugs into extravascular tumor tissue by increasing tumor vascular permeability through interaction with neuropilin (NRP). It is also known as iRGD, which is a prototypic tumor-penetrating CendR peptide. The peptide has been shown to inhibit spontaneous metastasis in mice and has a potent anti-metastatic effect. It works by binding to the NRP-1 receptor and inhibiting tumor cell migration and chemorepulsion. The peptide induces a collapse of cellular processes and partial cell detachment, resulting in the repellent activity. The anti-metastatic activity of iRGD may provide a significant additional benefit when this peptide is used for drug delivery to tumors.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Sugahara KN, Braun GB, de Mendoza TH, Kotamraju VR, French RP, Lowy AM, Teesalu T, Ruoslahti E. Tumor-penetrating iRGD peptide inhibits metastasis. Mol Cancer Ther. 2015 Jan;14(1):120-8. doi: 10.1158/1535-7163.MCT-14-0366. Epub 2014 Nov 12. PMID: 25392370; PMCID: PMC4297196.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.