Recombinant INT5 protein (His tag)

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Recombinant Human Integrator Complex Subunit 5 (Int5) Fragment (residues 2-252) is a high-quality protein product designed for structural and biochemical research. This 251-amino acid fragment, derived from the INTS5 gene, represents a stable and soluble unit ideal for studying the integrator complex's role in RNA polymerase II transcription termination and quality control mechanisms.

Integrator Complex Subunit 5 (Int5) is a crucial component of the multiprotein integrator complex that regulates RNA polymerase II (Pol II) transcription termination in the promoter-proximal region of genes. The complex serves as a quality checkpoint during transcription elongation by driving premature termination of unfavorably configured transcripts through three primary mechanisms: catalyzing dephosphorylation of Pol II's C-terminal domain (CTD) and SUPT5H/SPT5, degrading exiting nascent RNA transcripts via endonuclease activity, and promoting Pol II release from DNA. Additionally, the integrator complex terminates synthesis of various non-coding Pol II transcripts, including enhancer RNAs, small nuclear RNAs, telomerase RNAs, and long non-coding RNAs. Int5 also mediates recruitment of cytoplasmic dynein to the nuclear envelope, likely as part of the integrator complex. The protein localizes to the nucleus, cytoplasm, and nuclear membrane, where it functions as a multi-pass membrane protein. The provided fragment (residues 2-252) was computationally selected to represent a stable, soluble protein unit suitable for detailed structural and functional analyses.