Recombinant INT4 protein (His tag)

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Recombinant Integrator Complex Subunit 4 (Int4) Fragment (aa 42-323) is a high-quality protein product derived from Homo sapiens, designed for structural and biochemical studies. This 282-amino acid fragment represents a stable, soluble region of INTS4, optimized for research applications investigating transcriptional regulation and RNA polymerase II termination mechanisms.

Integrator Complex Subunit 4 (INTS4) is a crucial component of the integrator complex, a multiprotein assembly that regulates RNA polymerase II (Pol II) transcription termination in the promoter-proximal region of genes. The complex serves as a quality checkpoint during transcription elongation by driving premature termination of unfavorably configured transcripts through multiple mechanisms: dephosphorylation of Pol II's C-terminal domain (CTD) and SUPT5H/SPT5, degradation of nascent RNA via endonuclease activity, and promotion of Pol II release from DNA. Beyond protein-coding genes, the integrator complex terminates non-coding Pol II transcripts including enhancer RNAs, small nuclear RNAs, telomerase RNAs, and long non-coding RNAs. Within the complex, INTS4 functions as a structural scaffold that links INTS9 and INTS11 subunits. The protein localizes primarily to the nucleus, with additional presence in the cytoplasm, where it may mediate recruitment of cytoplasmic dynein to the nuclear envelope.