Recombinant INT3 protein (His tag)

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Recombinant Integrator complex subunit 3 (Int3) fragment (amino acids 34-499) from Homo sapiens, a key protein involved in RNA polymerase II transcription termination and DNA damage response. This 466-amino acid fragment is engineered for high stability and solubility, making it an ideal reagent for structural studies, biochemical assays, and functional investigations of transcriptional regulation and DNA repair mechanisms.

Integrator complex subunit 3 (INTS3) is a multifunctional protein that serves as a critical component of both the integrator complex and the SOSS complex. Within the integrator complex, INTS3 facilitates premature termination of RNA polymerase II transcription by participating in dephosphorylation of polymerase subunits, degradation of nascent RNA transcripts, and release of Pol II from DNA. This complex acts as a quality checkpoint during transcription elongation and terminates various non-coding RNAs including enhancer RNAs, snRNAs, and long non-coding RNAs. Additionally, INTS3 functions in the SOSS complex where it promotes DNA repair and G2/M checkpoint activation by associating with single-stranded DNA at lesion sites, supporting homologous recombination repair of double-strand breaks and ATM-dependent signaling pathways. The protein localizes primarily to the nucleus, forming distinct foci at DNA damage sites, with additional presence in the cytoplasm where it mediates recruitment of cytoplasmic dynein to the nuclear envelope. These diverse roles position INTS3 as a crucial regulator of transcriptional fidelity and genomic stability.