Hymenochirin-1Pa peptide

Hymenochirin-1Pa peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

LKLSPKTKDTLKKVLKGAIKGAIAIASMA

Leu-Lys-Leu-Ser-Pro-Lys-Thr-Lys-Asp-Thr-Leu-Lys-Lys-Val-Leu-Lys-Gly-Ala-Ile-Lys-Gly-Ala-Ile-Ala-Ile-Ala-Ser-Met-Ala

29

97.1%

Synthetic

Powder

Active against a broad spectrum of pathogens, including multiple S. aureus, S. epidermidis, A. baumannii strains, E. coli ATCC 25726, S. maltophilia (MIC 0.6-5 uM), K. pneumoniae ATCC 700603 (MIC 10 uM), E. faecalis ATCC 29212, P. aeruginosa ATCC 27853 (MIC 20 uM), and weakly against C. albicans (MIC 80 uM). Also active against human non-small lung adenocarcinoma A549 cells, breast adenocarcinoma MDA-MB-231 cells, and colorectal adenocarcinoma HT-29 cells. Its applications as antibacterial and anticancer agents may be limited due to hemolytic ability. However, the D9K mutant is not hemolytic. In TFE, residues 6-17 and 21-28 are helical (Serra et al. 2014). Reg. 09/23/2014. The source of Hymenochirin-1Pa is skin secretions, Merlin's clawed frog, Pseudhymenochirus merlini, Africa.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Conlon JM, Prajeep M, Mechkarska M, et al. Characterization of the host-defense peptides from skin secretions of Merlin's clawed frog Pseudhymenochirus merlini: insights into phylogenetic relationships among the Pipidae. Comp Biochem Physiol Part D Genomics Proteomics. 2013;8(4):352-7.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.