Hylin a1 peptide

Hylin a1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

IFGAILPLALGALKNLIK

Ile-Phe-Gly-Ala-Ile-Leu-Pro-Leu-Ala-Leu-Gly-Ala-Leu-Lys-Asn-Leu-Ile-Lys

18

97.9%

Synthetic

Powder

C-terminal amidation. Active against S. aureus (MIC 8 uM), P. aeruginosa (MIC 64 uM), E. coli (MIC 32 uM), B. subtilis (MIC 8 uM), and E. faecalis (MIC 16 uM). Addition of an acidic residue to the N-terminus disrupted its activity against Gram- bacteria. Thus, the N-terminus appears to determine bacteria specificity (Biopolymers 2010 in press). Updated 4/2014. Hylin a1 has Antibacterial, Antifungal activity. The source of Hylin a1 is South America, Hypsiboas albopunctatus.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides

• Castro MS, Ferreira TC, Cilli EM, et al. Hylin a1, the first cytolytic peptide isolated from the arboreal South American frog Hypsiboas albopunctatus ("spotted treefrog"). Peptides. 2009;30(2):291-6.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.