human Histatin 5 peptide

human Histatin 5 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

DSHAKRHHGYKRKFHEKHHSHRGY

Asp-Ser-His-Ala-Lys-Arg-His-His-Gly-Tyr-Lys-Arg-Lys-Phe-His-Glu-Lys-His-His-Ser-His-Arg-Gly-Tyr

24

98.3%

C₁₃₃H₁₉₅N₅₁O₃₃

3036.2

Synthetic

Powder

Active against C. albicans. Histatin 5 is a proteolytic product of histatin 3. The active domain of histitin 5 (Dh-5) corresponding to residues 11-24 showed HIV inhibitory effects. However,the results with the Dh-5 mutants are mixed (Groot et al. 2006 J. Virol. 80: 9236-9243). The sequence of Dhvar4 is KRLFKKLLFSLRKY, a helical peptide which is active against bacteria and fungi. Dhvar5 has a sequence LLLFLLKKRKKRKY, which is active against bacteria and fungi (Ruissen AL et al 2002 Peptides 23:1391-9). P-113 (P113) is a 12-residue active peptide corresponding to residues 4-15 of histatin 5 (Rothstein DM et al. 2001 AAC 45: 1367). Both the D and L forms (D=L) were equally active against C. albicans, but only the D-form (P113-D) was active in vivo (Sajjan US et al 2001 Antimicrob Agents Chemother 45:3437-3444). The His-His sequence is a good ligand for Cu2+ (Kulon K et al. 2008 J Inorg Biochem 102: 960-72). Enzyme inhibitor: Hst 5 is a competitive cysteine proteinase inhibitor (Gusman H et al., 2001).

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Oppenheim FG, Xu T, Mcmillian FM, et al. Histatins, a novel family of histidine-rich proteins in human parotid secretion. Isolation, characterization, primary structure, and fungistatic effects on Candida albicans. J Biol Chem. 1988;263(16):7472-7.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.