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Product Name
Human EGFR/HER1/ErbB1 (His & GST Tag) recombinant protein
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Description
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
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Protein name
Epidermal growth factor receptor
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Protein short names
PIG61; MENA; HER1; ERBB-1; 9030024J15RIK; WA5; ERBB1; ERRP; WA2; NISBD2; ERBB; ERRB1; RP23-295E4.1; WA-2; AI552599; EGFR
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Uniprot ID
P00533
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Gene Name
EGFR; ERBB; ERBB1; HER1
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Source/Expression Host
Baculovirus-Insect cells
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Expression Plasmid/cDNA
A DNA sequence encoding the cytoplasmic domain (Met 668-Ala 1210) of human EGFR (NP_005219) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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Protein Species
Human
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Molecular weight
The recombinant human EGFR /GST chimera consists of 780 amino acids and has a calculated molecular mass of 89.1 kDa.
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Purity
> 85 % as determined by SDS-PAGE
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Activity
The specific activity was determined to be 105 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate (see Activity Assay Protocol)
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Validations
Human EGFR / HER1 / ErbB1 (aa 668-1210) Protein (His & GST Tag) SDS-PAGE
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"