Human Defensin-5 (1-9) peptide

Human Defensin-5 (1-9) peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-ATCYCRTGR-NH2

H-Ala-Thr-Cys-Tyr-Cys-Arg-Thr-Gly-Arg-NH2

9

95.33%

C₄₀H₆₈N₁₆O₁₂S₂

1029.19

Synthetic

Powder

Human alpha-defensin(HD-5) is a strong activity antimicrobial peptide. HD-5(1–9) treatment affects certain bacterial genera and transiently increases microbial diversity in the small intestine. Mice treated orally with HD-5(1–9) had an increased amount of Akkermansia sp. compared with nontreated mice. Moreover, in contrast to other bacteria, Akkermansia muciniphila was not susceptible to HD-5(1–9) in a turbidity broth assay. MS showed that Human Defensin-5 (1-9) had a M.W.=1029.45 dalton

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Ehmann D, Wendler J, Koeninger L, et al. Paneth cell u03b1-defensins HD-5 and HD-6 display differential degradation into active antimicrobial fragments. Proc Natl Acad Sci U S A. 2019;116(9):3746-3751

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.