HRV-3C Protease (GST Tag)

HRV-3C Protease (GST Tag)

• DataSheet
• Material Safety Data Sheets (MSDS)

HRV 3C Protease (Human Rhinovirus 3C Protease) refers to the 3C protease of Human Rhinovirus (HRV), which recognizes and cleaves the specific amino‑acid sequence: Leu‑Glu‑Val‑Leu‑Phe‑Gln↓Gly‑Pro (where "↓" indicates the cleavage site). This recombinant HRV‑3C protease has a molecular weight of approximately 46 kDa and carries an N‑terminal GST tag. HRV 3C protease exhibits activity across a wide range of pH values, ionic strengths and temperatures. It retains high activity even at 0 °C, making it an ideal choice for temperature‑sensitive proteins.

Human Rhinovirus 3C Protease

E.coli

DNA encoding 1-182 aa were fused with N-terminal GST tag.

Human

Predictes a molecular mass of 46.28 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 46 kDa band.

>95%, by SDS-PAGE with Coomassie Brilliant Blue staining.

5U/μL. One unit (1 U) is defined as the amount of enzyme required to cleave 95% of 100 μg fusion protein in 25 mM Tris‑HCl buffer (pH 8.0) within 12–16 h at 4 °C.