Histatin 3 peptide

Histatin 3 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-DSHAKRHHGYKRKFHEKHHSHRGYRSNYLYDN-OH

H-Asp-Ser-His-Ala-Lys-Arg-His-His-Gly-Tyr-Lys-Arg-Lys-Phe-His-Glu-Lys-His-His-Ser-His-Arg-Gly-Tyr-Arg-Ser-Asn-Tyr-Leu-Tyr-Asp-Asn-OH

32

95.68%

C₁₇₈H₂₅₈N₆₄O₄₈

4062.33

Synthetic

Powder

Histatin 3 is a 32 amino acid histidine-rich peptide found in human saliva. Histatins function as antimicrobial peptides and are important components of the innate immune system. Histatin 3 has broad spectrum antimicrobial action including activity against gram positive and gram negative bacteria, fungi including Candida albicans and against the human protozoan parasite Leishania. Histatin 3 is post-translationally cleaved to form Histatin 5 and Histatin 8

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Fitzgerald et al (2003) Binding, internalisation and degradation of histatin 3 in histatin-resistant derivatives of Candida albicans. FEMS Microbiol. Lett. 220(2) 247 PMID: 12670688
• Oudhoff et al (2008) Histatins are the major wound-closure stimulating factors in human saliva as identified in a cell culture assay. FASEB J. 22(11) 3805 PMID: 18650243
• Hemadi et al (2017) Salivary proteins and microbiota as biomarkers for early childhood caries risk assessment. Int. J. Oral Sci.9, e1 PMID: 29125139

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.