Hexa-D-arginine peptide

Hexa-D-arginine peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-RRRRRR-NH2 (All D-type amino acides)

H-Arg-Arg-Arg-Arg-Arg-Arg-NH2 (All D-type amino acides)

6

95.29%

C₃₆H₇₅N₂₅O₆

954.14

Synthetic

Powder

Hexa-D-arginine is an inhibitor of furin, with Ki values of 106 nM, 580 nM and 13.2 μM for furin, PACE4 and PC1 respectively. Pseudomonas aeruginosa exotoxin A (PEA) protein requires furin-mediated cleavage for manifestation of toxicity and hexa-d-arginine effectively blocks PEA-induced cell lysis and is itself noncytotoxic. Administration of hexa-d-arginine to PEA-treated mice significantly improves their survival rate and decreases circulating levels of tumour necrosis factor alpha. Hexa-D-arginine also blocks furin mediated SARS-CoV-2 spike protein cleavage to suppress virus production and cytopathic effects.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Cameron et al (2000) Polyarginines are potent furin inhibitors. J.Biol.Chem. 275 36741 PMID: 10958789
• Sarac et al (2002) The furin inhibitor hexa-D-arginine blocks the activation of Pseudomonas aeruginosa exotoxin A in vivo. Infect Immun. 70(12) 7136 PMID: 12438396
• Cheng et al (2020) Furin Inhibitors Block SARS-CoV-2 Spike Protein Cleavage to Suppress Virus Production and Cytopathic Effects. Cell Rep. 33(2) 108254 PMID: 33007239

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.