Herpes Virus Inhibitor 1 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Herpes Virus Inhibitor 1 (HVI-1) is a synthetic nonapeptide with the sequence YAGAVVNDL, corresponding to the carboxyl-terminal nine amino acids of the small subunit of herpes simplex virus type 1 ribonucleotide reductase. This peptide specifically inhibits the viral enzyme by competing with the R2 subunit for binding to the R1 subunit, thereby disrupting the formation of the active R1/R2 dimer complex. The inhibitory activity is selective for herpesvirus ribonucleotide reductases, as their R2 carboxyl termini share homology that differs substantially from reductases of other species.

To facilitate intracellular delivery, the nonapeptide has been genetically linked to the B subunit of Escherichia coli heat-labile enterotoxin (EtxB), which binds to GM1 ganglioside receptors on eukaryotic cell surfaces and enters cells via receptor-mediated uptake. The resulting fusion protein retains the ability to assemble into a stable pentamer, binds specifically to the HSV-1 R1 subunit, and this interaction can be competitively disrupted by the free nonapeptide. This carrier-mediated approach enables the evaluation of the peptide's intracellular activity against viral ribonucleotide reductase in cell culture systems.

• Marcello A, Loregian A, Cross A, Marsden H, Hirst TR, Palù G. Specific inhibition of herpes virus replication by receptor-mediated entry of an antiviral peptide linked to Escherichia coli enterotoxin B subunit. Proc Natl Acad Sci U S A. 1994 Sep 13;91(19):8994-8. doi: 10.1073/pnas.91.19.8994. PMID: 8090758; PMCID: PMC44733.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.