GRKKRRQRRRPQRWEKISIINFEKL peptide

GRKKRRQRRRPQRWEKISIINFEKL peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GRKKRRQRRRPQRWEKISIINFEKL-OH

H-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Gln-Arg-Trp-Glu-Lys-Ile-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu-OH

25

95.81%

C₁₄₄H₂₄₈N₅₄O₃₄

3279.84

Synthetic

Powder

GRKKRRQRRRPQRWEKISIINFEKL is a peptide that was used in the study to coat the viral envelope of oncolytic viruses. It was complexed with vaccinia virus and herpes simplex virus 1 (HSV-1) to enhance the immune response against tumor antigens. The peptide was attached to the viral envelope using a cell-penetrating peptide sequence derived from the human immunodeficiency virus Tat or conjugated with cholesterol. The coating of the viral envelope with GRKKRRQRRRPQRWEKISIINFEKL did not have any adverse effects on the oncolytic efficacy of the viruses and did not show any toxicity towards the tested cell lines. The peptide-coated viruses were found to significantly enhance anti-tumor immunity and increase the number of tumor-specific CD8+ T cells in the tumor microenvironment.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Ylösmäki E, Malorzo C, Capasso C, Honkasalo O, Fusciello M, Martins B, Ylösmäki L, Louna A, Feola S, Paavilainen H, Peltonen K, Hukkanen V, Viitala T, Cerullo V. Personalized Cancer Vaccine Platform for Clinically Relevant Oncolytic Enveloped Viruses. Mol Ther. 2018 Sep 5;26(9):2315-2325. doi: 10.1016/j.ymthe.2018.06.008. Epub 2018 Jun 19. PMID: 30005865; PMCID: PMC6127500.

Trifluoroacetic acid (TFA) has a significant impact on peptides due to its role in the peptide synthesis process.

TFA is essential for the protonation of peptides that lack basic amino acids such as Arginine (Arg), Histidine (His), and Lysine (Lys), or ones that have blocked N-termini. As a result, peptides often contain TFA salts in the final product.

TFA residues, when present in custom peptides, can cause unpredictable fluctuations in experimental data. At a nanomolar (nM) level, TFA can influence cell experiments, hindering cell growth at low concentrations (as low as 10 nM) and promoting it at higher doses (0.5–7.0 mM). It can also serve as an allosteric regulator on the GlyR of glycine receptors, thereby increasing receptor activity at lower glycine concentrations.

In an in vivo setting, TFA can trifluoroacetylate amino groups in proteins and phospholipids, inducing potentially unwanted antibody responses. Moreover, TFA can impact structure studies as it affects spectrum absorption.