GRKKRRQRRRPQRWEKISIINFEKL peptide

GRKKRRQRRRPQRWEKISIINFEKL peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GRKKRRQRRRPQRWEKISIINFEKL-OH

H-Gly-Arg-Lys-Lys-Arg-Arg-Gln-Arg-Arg-Arg-Pro-Gln-Arg-Trp-Glu-Lys-Ile-Ser-Ile-Ile-Asn-Phe-Glu-Lys-Leu-OH

25

95.81%

C₁₄₄H₂₄₈N₅₄O₃₄

3279.84

Synthetic

Powder

GRKKRRQRRRPQRWEKISIINFEKL is a peptide that was used in the study to coat the viral envelope of oncolytic viruses. It was complexed with vaccinia virus and herpes simplex virus 1 (HSV-1) to enhance the immune response against tumor antigens. The peptide was attached to the viral envelope using a cell-penetrating peptide sequence derived from the human immunodeficiency virus Tat or conjugated with cholesterol. The coating of the viral envelope with GRKKRRQRRRPQRWEKISIINFEKL did not have any adverse effects on the oncolytic efficacy of the viruses and did not show any toxicity towards the tested cell lines. The peptide-coated viruses were found to significantly enhance anti-tumor immunity and increase the number of tumor-specific CD8+ T cells in the tumor microenvironment.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Ylösmäki E, Malorzo C, Capasso C, Honkasalo O, Fusciello M, Martins B, Ylösmäki L, Louna A, Feola S, Paavilainen H, Peltonen K, Hukkanen V, Viitala T, Cerullo V. Personalized Cancer Vaccine Platform for Clinically Relevant Oncolytic Enveloped Viruses. Mol Ther. 2018 Sep 5;26(9):2315-2325. doi: 10.1016/j.ymthe.2018.06.008. Epub 2018 Jun 19. PMID: 30005865; PMCID: PMC6127500.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.