Gly-Arg-AMC peptide

Gly-Arg-AMC peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GR-AMC

H-Gly-Arg-AMC

2

95.66%

C₁₈H₂₄N₆O₄

388.42

65147-19-5

493582

Synthetic

Powder

The peptide Gly-Tyr-AMC (glycyl-tyrosyl-amidomethylcoumarin) is a dipeptide substrate used in the study to investigate the catalytic properties of cathepsin C. Cathepsin C is a lysosomal cysteine protease that sequentially removes dipeptides from the N-termini of protein and peptide substrates. The kinetic parameters for Gly-Tyr-AMC were determined through steady-state and pre-steady-state kinetic studies. It was found that Gly-Tyr-AMC is a less active substrate compared to Gly-Arg-AMC and Ser-Tyr-AMC, with a kcat of 28 ± 1 s^(-1) and a kcat / Ka of 0.49 ± 0.067 μM^(-1) s^(-1).

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Rubach JK, Cui G, Schneck JL, Taylor AN, Zhao B, Smallwood A, Nevins N, Wisnoski D, Thrall SH, Meek TD. The amino-acid substituents of dipeptide substrates of cathepsin C can determine the rate-limiting steps of catalysis. Biochemistry. 2012 Sep 25;51(38):7551-68. doi: 10.1021/bi300719b. Epub 2012 Sep 13. PMID: 22928782.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.