GH12 peptide

GH12 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-GLLWHLLHHLLH-NH2

H-Gly-Leu-Leu-Trp-His-Leu-Leu-His-His-Leu-Leu-His-NH2

12

95.83%

C₇₃H₁₁₀N₂₂O₁₂

1487.79

Synthetic

Powder

GH12 (GLLWHLLHHLLH-NH2) is an amphipathic α-helical antimicrobial peptide against oral streptococci in vitro. The peptide showed bactericidal activity against oral streptococci, with minimum inhibitory concentrations ranging from 6.7 to 32.0 μg/ml. GH12 concentrations 4-fold higher than the minimum bactericidal concentration completely killed oral streptococci within 20 min. Treating oral streptococci with GH12 caused noticeable changes in bacterial viability and morphology based on confocal laser scanning microscopy and scanning electron microscopy.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Wang, Y., Fan, Y., Zhou, Z., Tu, H., Ren, Q., Wang, X., Ding, L., Zhou, X., and Zhang, L. (2017) De novo Synthetic Short Antimicrobial Peptides against Cariogenic Bacteria. Arch. Oral Biol. 80, 41-50.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.