Foxy 5 peptide

Foxy 5 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Formylation-MDGCEL-OH

Formylation-Met-Asp-Gly-Cys-Glu-Leu-OH

6

95.04%

C₂₆H₄₂N₆O₁₂S₂

694.77

Synthetic

Powder

Foxy 5 is a formylated hexapeptide derived from the sequence of the Wnt5a ligand which acts as a Wnt5a mimetic. Foxy 5 is able to mimic important effects exerted by the full Wnt5a molecule such as binding to the Frz-5 receptor, and reproducing Wnt5a induced inhibition of human breast cancer cell migration in vitro. Foxy 5 significantly reduces the metastatic spread of WNT5a low DU145 prostate cancer cells in an in vivo orthotopic xenograft mouse model.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Säfholm et al (2006) A formylated hexapeptide ligand mimics the ability of Wnt-5a to impair migration of human breast epithelial cells. J.Biol.Chem. 281 2740 PMID: 16330545
• Säfholm et al (2008) The Wnt-5a-derived hexapeptide Foxy-5 inhibits breast cancer metastasis in vivo by targeting cell motility. Clin.Cancer Res. 14 6556 PMID: 18927296
• Canesin (2017) Treatment with the WNT5A-mimicking peptide Foxy-5 effectively reduces the metastatic spread of WNT5A-low prostate cancer cells in an orthotopic mouse model. PLoS One 12(9) e0184418 PMID: 28886116

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.