FLAG Epitope Peptide, DYKDDDDK

FLAG Epitope Peptide, DYKDDDDK

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

DYKDDDDK

H-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-OH

8

95.03%

C₄₁H₆₀N₁₀O₂₀

1012.96

98849-88-8

Synthetic

Powder

The FLAG Epitope Peptide consists of eight amino acids (DYKDDDDK) and represents a versatile fusion tag for the purification of recombinant proteins. It can be enzymatically removed with enterokinase which cleaves C-terminally to the tag sequence. The peptide is commonly used for the elution of immunoaffinity chromatography-purified recombinant proteins.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• T.P.Hopp et al., Nat. Biotechnol., 6, 1204 (1988)
• T.M.Shih and A.L.Goldin, J. Cell Biol., 136, 1037 (1997)
• A.Einhauer and A.Jungbauer, J. Biochem. Biophys. Methods, 49, 455 (2001)
• K.Y.Johnson et al., Biotechniques, 32, 1270 (2002)
• A.Einhauer and A.Jungbauer, J. Chromatogr. A., 1009, 81 (2003)

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.