Fc-III-4C peptide

Fc-III-4C peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-CDCAWHLGELVWCTC-OH (C1-C15, C3-C13 disulfide bridges)

H-Cys-Asp-Cys-Ala-Trp-His-Leu-Gly-Glu-Leu-Val-Trp-Cys-Thr-Cys-OH (C1-C15, C3-C13 disulfide bridges)

15

95.05%

C₇₅H₁₀₃N₁₉O₂₁S₄

1734.99

Synthetic

Powder

The Fc-III-4C peptide is an affinity tag that was developed for recombinant protein expression and purification. It is created by adding two cysteine residues onto the N- and C-termini of the Fc-III peptide, resulting in four cysteine residues that form two disulfide linkages. The Fc-III-4C tag has a higher binding affinity to human IgG compared to the Fc-III tag, with a measured Kd value of 2.28 nM.

The primary sequence of Fc-III peptide was DCAWHLGELVWCT (Cat.#: 319361), in which Cys 2 and Cys 12 form a disulfide linkage that make the peptide has a cyclic structure.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Gong Y, Yi M, Zhang L, Feng S, Deng H. Characterization of the Fc-III-4C-based recombinant protein expression system by using carbonic anhydrase as the model protein. Protein Expr Purif. 2021 Jan;177:105761. doi: 10.1016/j.pep.2020.105761. Epub 2020 Sep 19. PMID: 32956801.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.