EK1 peptide

EK1 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-SLDQINVTFLDLEYEMKKLEEAIKKLEESYIDLKEL-OH

H-Ser-Leu-Asp-Gln-Ile-Asn-Val-Thr-Phe-Leu-Asp-Leu-Glu-Tyr-Glu-Met-Lys-Lys-Leu-Glu-Glu-Ala-Ile-Lys-Lys-Leu-Glu-Glu-Ser-Tyr-Ile-Asp-Leu-Lys-Glu-Leu-OH

36

90.82%

C₁₉₆H₃₁₇N₄₃O₆₄S

4331.9

Synthetic

Powder

EK1 was effective in inhibiting infection by all HCoVs tested, including SARS-CoV, MERS-CoV, HCoV-OC43, HCoV-229E, and HCoV-NL63, as well as some bat SARSr-CoVs, such as SARSr-CoV-WIV1, SARSr-CoV-Rs3367, and SARSr-CoV-SHC014. Our mechanistic study showed that EK1 bound to the HR1 domain of an HCoV and blocked 6-HB formation between the viral HR1 and HR2 domains, resulting in the inhibition of viral fusion and entry into the host cell.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Zhou J, Xu W, Liu Z, Wang C, Xia S, Lan Q, Cai Y, Su S, Pu J, Xing L, Xie Y, Lu L, Jiang S, Wang Q. A highly potent and stable pan-coronavirus fusion inhibitor as a candidate prophylactic and therapeutic for COVID-19 and other coronavirus diseases. Acta Pharm Sin B. 2022 Apr;12(4):1652-1661. doi: 10.1016/j.apsb.2021.07.026. Epub 2021 Aug 2. PMID: 34367893; PMCID: PMC8327648.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.