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Recombinant EGLN2 protein (His tag)

Cat.#: 547999

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Product Information

  • Product Name
    Recombinant EGLN2 protein (His tag)
  • Documents
  • Description

    Recombinant Human Prolyl Hydroxylase EGLN2 (EC 1.14.11.29) Fragment (aa 169-407) is a high-purity protein fragment encompassing the essential Fe2OG dioxygenase domain. This product is an invaluable tool for investigating oxygen-sensing mechanisms, HIF-alpha regulation, and prolyl hydroxylation in normoxic and hypoxic conditions, offering researchers a focused reagent for domain-specific functional studies.

    EGLN2, also known as HIF-prolyl hydroxylase 1 (PHD1), is a crucial cellular oxygen sensor that catalyzes the hydroxylation of proline residues in target proteins under normoxic conditions. It primarily targets hypoxia-inducible factor alpha subunits (HIF1A and HIF2A) via recognition of LXXLAP motifs, promoting their degradation via the von Hippel-Lindau ubiquitination pathway. This regulation is vital for hypoxia tolerance, apoptosis in muscle tissues, and neuronal survival. Beyond HIF signaling, EGLN2 hydroxylates diverse substrates including ATF4, IKBKB, and CEP192, linking oxygen sensing to NF-k

  • Protein name
    Prolyl hydroxylase EGLN2
  • Uniprot ID
    Q96KS0
  • Gene Name
    EGLN2; EIT6
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    DNA encoding 169-407 aa (Q96KS0) were fused with N-terminal 6His tag.
  • Sequence 
    MSKIKHHHHH HSSGALPSAP ERLALDYIVP CMRYYGICVK DSFLGAALGG RVLAEVEALK RGGRLRDGQL VSQRAIPPRS
IRGDQIAWVE GHEPGCRSIG ALMAHVDAVI RHCAGRLGSY VINGRTKAMV ACYPGNGLGY VRHVDNPHGD GRCITCIYYL
NQNWDVKVHG GLLQIFPEGR PVVANIEPLF DRLLIFWSDR RNPHEVKPAY ATRYAITVWY FDAKERAAAK DKYQLASGQK
GVQVPVSQPP TPT
  • Protein Species
    Human
  • Molecular weight
    Predictes a molecular mass of 28.02 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 28 kDa band.
  • Purity
    >85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
  • Activity
    Not tested.
  • Validations

    Recombinant EGLN2 protein (His tag) SDS-PAGE

    Recombinant EGLN2 protein (His tag) SDS-PAGE

    M: Marker; Lane 1: EGLN2; Lane 2: BSA

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"