EcDBS1R5 peptide

EcDBS1R5 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-PMKKLKLALRLAAKIAPVW-OH

H-Pro-Met-Lys-Lys-Leu-Lys-Leu-Ala-Leu-Arg-Leu-Ala-Ala-Lys-Ile-Ala-Pro-Val-Trp-OH

19

95.78%

C₁₀₃H₁₇₉N₂₇O₂₀S

2147.75

Synthetic

Powder

The computationally generated peptide, EcDBS1R5 (PMKKLKLALRLAAKIAPVW), inspired by an E. coli AMP, was active at 8 μmol L−1 against P. aeruginosa in in vitro assays, while in a skin scarification mouse model infected by P. aeruginosa, the same peptide was active only at a concentration 8-fold higher (64 μmol L−1), even though the concentration was lower than its cytotoxic activity (>128 μmol L−1).

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Cardoso, M. H., Cândido, E. S., Chan, L. Y., Der Torossian Torres, M., Oshiro, K. G. N., Rezende, S. B., Porto, W. F., Lu, T. K., de la Fuente-Nunez, C., Craik, D. J., and Franco, O. L. A (2018) Computationally Designed Peptide Derived from Escherichia coli as a Potential Drug Template for Antibacterial and Antibiofilm Therapies. ACS Infect. Dis. 4, 1727-1736.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.