eCATH-2 peptide

eCATH-2 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

KRRHWFPLSFQEFLEQLRRFRDQLPFP

Lys-Arg-Arg-His-Trp-Phe-Pro-Leu-Ser-Phe-Gln-Glu-Phe-Leu-Glu-Gln-Leu-Arg-Arg-Phe-Arg-Asp-Gln-Leu-Pro-Phe-Pro

27

95.8%

Synthetic

Powder

present in uncleaved forms in horse neutrophils. active against a broad range of bacteria such as E. coli ATCC 25922, E. coli ML 35 (MIC 14 ug/ml), S. marcescens ATCC 8100, K. pneumoniae ATCC 13883 (MIC 28.5), S. aureus ATCC 25923 (MIC 57 ug/ml), S. epidermidis ATCC 12228 (MIC 28.5 ug/ml), and B. megaterium Bm11 (MIC 14 ug/ml). Active against fungi various C. neoformans, R. rubra (MIC 21-57 ug/ml) in RPMI medium (Skerlavaj B et al., 2001). Updated 7/8/2013. eCATH-2 has Antimicrobial activity. The source of eCATH-2 is myeloid cells, Horse Equus asinus.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Scocchi M, Bontempo D, Boscolo S, Tomasinsig L, Giulotto E, Zanetti M. Novel cathelicidins in horse leukocytes(1). FEBS Lett. 1999;457(3):459-64.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.