eCATH-1 peptide

eCATH-1 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

KRFGRLAKSFLRMRILLPRRKILLAS

Lys-Arg-Phe-Gly-Arg-Leu-Ala-Lys-Ser-Phe-Leu-Arg-Met-Arg-Ile-Leu-Leu-Pro-Arg-Arg-Lys-Ile-Leu-Leu-Ala-Ser

26

96.8%

31409

Synthetic

Powder

Potently active against a broad range of bacteria such as E. coli ATCC 25922, E. coli ML 35, P. aeruginosa ATCC 27853, S. marcescens ATCC 8100, K. pneumoniae ATCC 13883, S. aureus ATCC 25923, MRSA, S. epidermidis ATCC 12228, S. equinus, and B. megaterium Bm11 (MIC 3-50 ug/ml). Active also against fungi various C. neoformans, R. rubra (MIC 3-12.5 ug/ml) in RPMI medium and Sabouraud medium (50 ug/ml for R. rubra in Sabouraud medium) (Skerlavaj B et al., 2001). Animal model:mouse, the equine antimicrobial peptide eCATH1 is effective against the facultative intracellular pathogen Rhodococcus equi (R. equi) in mice (Schlusselhuber M et al., 2013). Updated 7/8/2013. eCATH-1 has Antimicrobial activity. eCATH-1 was found in Horse Equus asinus.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Scocchi M, Bontempo D, Boscolo S, Tomasinsig L, Giulotto E, Zanetti M. Novel cathelicidins in horse leukocytes(1). FEBS Lett. 1999;457(3):459-64.
• Cauchard S, Van Reet N, Büscher P, Goux D, Grötzinger J, Leippe M, Cattoir V, Laugier C, Cauchard J. Killing of Trypanozoon Parasites by the Equine Cathelicidin eCATH1. Antimicrob Agents Chemother. 2016 Apr 22;60(5):2610-9.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.