-
Product Name
Recombinant E4F1 protein (His tag)
- Documents
-
Description
This product features a recombinant fragment of the human Transcription factor E4F1 (E4F1), spanning residues 434-596. This 163-amino acid fragment has been computationally designed for optimal stability and solubility, making it an ideal tool for structural studies and biochemical assays investigating E4F1's roles in transcription, cell cycle regulation, and ubiquitin ligase activity.
Transcription factor E4F1 is a multifunctional protein involved in transcriptional repression and ubiquitin ligase activity, specifically mediating ubiquitination of chromatin-associated TP53. It plays critical roles in cell survival, proliferation, and regulation of the cell cycle through the p53 and pRB tumor suppressor pathways, as well as controlling cyclin CCNA2 transcription. E4F1 also serves as a cellular target for the adenoviral oncoprotein E1A, contributing to both activation and repression of viral genes. The protein is primarily localized in the nucleus and nucleoplasm, with a small fraction detected in the
-
Protein name
Transcription factor E4F1
-
Uniprot ID
Q66K89
-
Gene Name
E4F1; E4F
-
Source/Expression Host
E. coli
-
Expression Plasmid/cDNA
DNA encoding 434-596 aa (Q66K89) were fused with C-terminal 6His tag.
-
Sequence
MSKIKTHPCP QCSETFPTAA TLEAHKRGHT GPRPFACAQC GKAFPKAYLL KKHQEVHVRE RRFRCGDCGK LYKTIAHVRG HRRVHSDERP YPCPKCGKRY KTKNAQQVHF RTHLEEKPHV CQFCSRGFRE KGSLVRHVRH HTGEKPFKCY KCGRGFAEHG TLNRHLRTLE HHHHHH
-
Protein Species
Human
-
Molecular weight
Predictes a molecular mass of 20.56 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 21 kDa band.
-
Purity
>85%, by SDS-PAGE with Coomassie Brilliant Blue staining.
-
Activity
Not tested.
-
Validations
Recombinant E4F1 protein (His tag) SDS-PAGE
M: Marker; Lane 1: E4F1; Lane 2: BSA
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"