Eα (52-68) peptide

Eα (52-68) peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

ASFEAQGALANIAVDKA

Ala-Ser-Phe-Glu-Ala-Gln-Gly-Ala-Leu-Ala-Asn-Ile-Ala-Val-Asp-Lys-Ala

17

95.37%

C₇₃H₁₁₈N₂₀O₂₅

1675.82

137756-45-7

Synthetic

Powder

Eα (52-68) is a specific peptide sequence with the amino acid composition ASFEAQGALANIAVDKA. It is derived from either the human HLA-DR alpha chain (Uniprot: P01903, corresponding to amino acids 77-93) or the mouse I-Eα chain, and is widely used in immunology research. Specifically, it serves as a high-quality epitope peptide (IEDB: 4397) for studying T cell responses and interactions with major histocompatibility complex (MHC) molecules, and is suitable for stimulating antigen-specific T cells in various T cell assays including ELISPOT, intracellular cytokine staining (ICS), cytotoxicity assays, and proliferation assays.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Trembleau S, Gregori S, Penna G, Gorny I, Adorini L. IL-12 administration reveals diabetogenic T cells in genetically resistant I-Ealpha-transgenic nonobese diabetic mice: resistance to autoimmune diabetes is associated with binding of Ealpha-derived peptides to the I-A(g7) molecule. J Immunol. 2001 Oct 1;167(7):4104-14. doi: 10.4049/jimmunol.167.7.4104. PMID: 11564833.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.