Drosocin peptide

Drosocin peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

GKPRPYSPRPTSHPRPIRV

Gly-Lys-Pro-Arg-Pro-Tyr-Ser-Pro-Arg-Pro-Thr-Ser-His-Pro-Arg-Pro-Ile-Arg-Val

19

97%

C₉₈H₁₆₀N₃₄O₂₄

2198.52

Synthetic

Powder

It is induced via the IMD pathway. Position T11 is o-glycosylated and important for full activity. Subsequent analysis showed that S7 may also be glycosylated. Turn elements occur near residues 4-7, 10-13, 17, and 18. At modorate concentrations, this peptide, as well as pyrrhocoricin (AP170), is also active against a few Gram-positive bacteria such as B. megaterium (44% homology to E. coli DnaK D-E helix) and M. luteus (Hoffmann R et al. 1999 Biochim Biophys Acta 1426:459-67). Mechanism of Action (MOA): It binds to an internal protein target DnaK (Eur J Biochem 2002; 269: 4226-4237). Note that all D-drosocin is inactive (so a non-membrane target) and the L form kills bacteria in hours not in minutes. Updated 12/2012 GW. Drosocin has Antibacterial activity. The source of Drosocin is Fruit fly, Drosophila melanogaster.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Bulet P, Dimarcq JL, Hetru C, et al. A novel inducible antibacterial peptide of Drosophila carries an O-glycosylated substitution. J Biol Chem. 1993;268(20):14893-7.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.