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Product Name
Recombinant DPH2 protein (His tag)
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Description
Recombinant Human DPH2 Protein Fragment (residues 1-489) is a high-purity, stable protein designed for structural and biochemical studies. This fragment represents the full-length 2-(3-amino-3-carboxypropyl)histidine synthase subunit 2, a key enzyme in diphthamide biosynthesis, and has been optimized for solubility and experimental reliability.
The full-length DPH2 protein (UniProt ID: Q9BQC3) is essential for the first step of diphthamide biosynthesis, a critical post-translational modification of histidine in elongation factor 2. DPH2, in complex with DPH1, transfers a 3-amino-3-carboxypropyl group from S-adenosyl-L-methionine (SAM) to histidine, a reaction supported by a reduction system involving DPH3 and an NADH-dependent reductase. DPH2 also facilitates the reduction of the iron-sulfur cluster in DPH1, underscoring its role in cellular processes related to protein synthesis and toxin resistance. The provided fragment (1-489) encompasses the complete functional domain, making it ideal for investigating diphthamide biosynthesis mechanisms.
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Protein name
2-
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Uniprot ID
Q9BQC3
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Gene Name
DPH2; DPH2L2
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Source/Expression Host
E. coli
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Expression Plasmid/cDNA
DNA encoding 1-489 aa (Q9BQC3) were fused with 6His tag.
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Protein Species
Human
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Molecular weight
Predictes a molecular mass of 53.74 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 54 kDa band.
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Purity
>89%, by SDS-PAGE with Coomassie Brilliant Blue staining.
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Activity
Not tested.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"