DP7 peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

DP7 is a synthetic antimicrobial peptide (AMP) derived from the parent peptide HH2 through an amino acid-based activity prediction method. This 12-mer peptide, with the sequence VQWRIRVAVIRK, exhibits broad-spectrum antimicrobial activity against both Gram-positive and Gram-negative bacterial strains. Compared to HH2, DP7 demonstrates enhanced antimicrobial efficacy while maintaining lower cytotoxicity, as evidenced by hemolysis and cell viability assays.

Electron microscopy studies reveal that DP7 disrupts the outer membrane of Staphylococcus aureus without causing cytoplasmic leakage, distinguishing its mechanism from typical pore-forming AMPs. The peptide's amphipathic and cationic properties facilitate selective binding to bacterial membranes, leading to structural damage and inhibition of bacterial growth. DP7 represents a rationally designed AMP with optimized activity and reduced host cell toxicity.

• Wu S, Yang Y, Wang S, Dong C, Zhang X, Zhang R, Yang L. Dextran and peptide-based pH-sensitive hydrogel boosts healing process in multidrug-resistant bacteria-infected wounds. Carbohydr Polym. 2022 Feb 15;278:118994. doi: 10.1016/j.carbpol.2021.118994. Epub 2021 Dec 10. PMID: 34973798.
• Wu X, Wang Z, Li X, Fan Y, He G, Wan Y, Yu C, Tang J, Li M, Zhang X, Zhang H, Xiang R, Pan Y, Liu Y, Lu L, Yang L. In vitro and in vivo activities of antimicrobial peptides developed using an amino acid-based activity prediction method. Antimicrob Agents Chemother. 2014 Sep;58(9):5342-9. doi: 10.1128/AAC.02823-14. Epub 2014 Jun 30. PMID: 24982064; PMCID: PMC4135812.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.