DLTAGLLE peptide

DLTAGLLE peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-DLTAGLLE-OH

H-Asp-Leu-Thr-Ala-Gly-Leu-Leu-Glu-OH

8

95.99%

C₃₆H₆₂N₈O₁₄

830.92

Synthetic

Powder

DLTAGLLE is a novel ACE-inhibitory peptide which was isolated and purified from Lepidotrigla microptera protein hydrolysate (LMPH) using various techniques such as ultrafiltration, gel filtration chromatography, and preparative high performance liquid chromatography (prep-HPLC). It has been identified as a noncompetitive inhibitor by Lineweaver-Burk plot. The amino acid sequence of the purified peptide is Phe-Leu-Thr-Ala-Gly-Leu-Leu-Asp. The IC50 value of DLTAGLLE was found to be 0.13 mg/mL. The peptide has ACE(Angiotensin Converting Enzyme)-inhibitory activity, which means it can help regulate blood pressure. It is stable across a range of temperatures (<100 • C) and pH values (3.0-11.0) and retained after gastrointestinal digestion. DLTAGLLE has the potential to develop functional food or novel ACE-inhibitor drugs.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Hu X, Dai Z, Jin R. Purification and Identification of a Novel Angiotensin Converting Enzyme Inhibitory Peptide from the Enzymatic Hydrolysate of Lepidotrigla microptera. Foods. 2022 Jun 26;11(13):1889. doi: 10.3390/foods11131889. PMID: 35804705; PMCID: PMC9265830.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.