Diazepam binding inhibitor-34 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

Diazepam binding inhibitor-34 (DBI-34), also known as TTN, is a 34-amino acid neuroactive peptide derived from the proteolytic cleavage of acyl-CoA-binding protein (ACBP). ACBP, a highly conserved protein with a primary intracellular role in fatty acid metabolism, is secreted from astrocytes via an unconventional, brefeldin A-insensitive pathway that involves components of the autophagy machinery. Following secretion, ACBP is rapidly processed extracellularly by a cell surface-associated protease activity to generate the bioactive DBI-34 peptide.

This peptide is classified as an endozepine and functions as an endogenous ligand for the GABA_A receptor. Its production in astrocytes can be rapidly induced within minutes by specific neurosteroids, such as pregnenolone sulfate and cortisol, as well as by cellular depolarization. The secretion and subsequent processing of ACBP into DBI-34 represent a conserved mechanism for intercellular signaling, highlighting a non-classical pathway for peptide generation and release in the central nervous system.

• Slobodyansky E, Guidotti A, Wambebe C, Berkovich A, Costa E. Isolation and characterization of a rat brain triakontatetraneuropeptide, a posttranslational product of diazepam binding inhibitor: specific action at the Ro 5-4864 recognition site. J Neurochem. 1989 Oct;53(4):1276-84. doi: 10.1111/j.1471-4159.1989.tb07425.x. PMID: 2769267.
• Loomis WF, Behrens MM, Williams ME, Anjard C. Pregnenolone sulfate and cortisol induce secretion of acyl-CoA-binding protein and its conversion into endozepines from astrocytes. J Biol Chem. 2010 Jul 9;285(28):21359-65. doi: 10.1074/jbc.M110.105858. Epub 2010 May 7. PMID: 20452969; PMCID: PMC2898429.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.