Dermcidin - DCD-1L peptide

Dermcidin - DCD-1L peptide

• COA/MS/HPLC Report
• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

H-SSLLEKGLDGAKKAVGGLGKLGKDAVEDLESVGKGAVHDVKDVLDSVL-OH

H-Ser-Ser-Leu-Leu-Glu-Lys-Gly-Leu-Asp-Gly-Ala-Lys-Lys-Ala-Val-Gly-Gly-Leu-Gly-Lys-Leu-Gly-Lys-Asp-Ala-Val-Glu-Asp-Leu-Glu-Ser-Val-Gly-Lys-Gly-Ala-Val-His-Asp-Val-Lys-Asp-Val-Leu-Asp-Ser-Val-Leu-OH

48

97.54%

C₂₁₀H₃₅₉N₅₇O₇₁

4818.41

Synthetic

Powder

DCD-1L is a dermcidin-derived peptide. It is constitutively expressed in eccrine sweat glands and secreted into sweat. It has an amino acid sequence of SSLLEKGLDGAKKAVGGLGKLGKDAVEDLESVGKGAVHDVKDVLDSVL with a net charge of -2 and a calculated pI of 5.07. DCD-1L shows antimicrobial activity against a variety of pathogenic microorganisms including Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Candida albicans, Staphylococcus epidermidis, Pseudomonas putida, methicillin-resistant S. aureus, rifampin- and isoniazid-resistant Mycobacterium tuberculosis, Listeria monocytogenes, and Salmonella enterica serovar Typhimurium. It is proteolytically processed from a 110-amino-acid precursor protein of dermcidin. In human eccrine sweat, DCD-1L and other DCD-derived peptides are generated by postsecretory proteolytic processing, and its activity may be related to the formation of stable complexes and interaction with the bacterial membrane, although it does not permeabilize the bacterial membrane like some other antimicrobial peptides. The alteration from anionic DCD-1L to cationic peptides during proteolytic processing may lead to a different spectrum of antimicrobial activity, and at least the first three amino acids SSL seem important for its antimicrobial function, with the active part of its sequence potentially residing in the first 23 amino acids.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Schittek B, Hipfel R, Sauer B, et al. Dermcidin: a novel human antibiotic peptide secreted by sweat glands. Nat Immunol. 2001;2(12):1133-7.

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.