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Recombinant DCAF1 protein (His tag)

Cat.#: 539304

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Product Information

  • Product Name
    Recombinant DCAF1 protein (His tag)
  • Documents
  • Description

    Recombinant Human DDB1- and CUL4-associated factor 1 (DCAF1/VprBP) Fragment (aa 1002-1394) – a highly stable and soluble protein fragment ideal for structural studies, biochemical assays, and functional investigations into E3 ubiquitin ligase complexes and serine/threonine kinase activities.

    DCAF1 (VprBP) is a multifunctional protein that serves as both a substrate recognition component of E3 ubiquitin-protein ligase complexes and an atypical serine/threonine-protein kinase. It plays critical roles in cell cycle regulation, telomerase inhibition through TERT degradation, histone modification via phosphorylation of H2A at Thr-120, and error-limiting repair during V(D)J recombination. The protein is involved in key cellular processes including B-cell development, JNK-mediated apoptosis, and oocyte maintenance. During viral infections, DCAF1 is hijacked by HIV-1 Vpr to induce G2 cell cycle arrest and by HIV-2 Vpx to enhance macrophage infection efficiency. The protein localizes to both cytoplasm and nuc

  • Protein name
    DDB1- and CUL4-associated factor 1
  • Uniprot ID
    Q9Y4B6
  • Gene Name
    DCAF1; KIAA0800; RIP; VPRBP
  • Source/Expression Host
    E. coli
  • Expression Plasmid/cDNA
    DNA encoding 1002-1394 aa (Q9Y4B6) were fused with C-terminal 6His tag.
  • Sequence 
    MSKIKPTLDS IITEYLREQH ARCKNPVATC PPFSLFTPHQ CPEPKQRRQA PINFTSRLNR RASFPKYGGV DGGCFDRHLI
FSRFRPISVF REANEDESGF TCCAFSARER FLMLGTCTGQ LKLYNVFSGQ EEASYNCHNS AITHLEPSRD GSLLLTSATW
SQPLSALWGM KSVFDMKHSF TEDHYVEFSK HSQDRVIGTK GDIAHIYDIQ TGNKLLTLFN PDLANNYKRN CATFNPTDDL
VLNDGVLWDV RSAQAIHKFD KFNMNISGVF HPNGLEVIIN TEIWDLRTFH LLHTVPALDQ CRVVFNHTGT VMYGAMLQAD
DEDDLMEERM KSPFGSSFRT FNATDYKPIA TIDVKRNIFD LCTDTKDCYL AVIENQGSMD ALNMDTVCRL YEVGRQRLLE
HHHHHH
  • Protein Species
    Human
  • Molecular weight
    Predictes a molecular mass of 46.42 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 47 kDa band.
  • Purity
    >90%, by SDS-PAGE with Coomassie Brilliant Blue staining.
  • Activity
    Not tested.
  • Validations

    Recombinant DCAF1 protein (His tag) SDS-PAGE

    Recombinant DCAF1 protein (His tag) SDS-PAGE

    M: Marker; Lane 1: DCAF1; Lane 2: BSA

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"