DC432 peptide

DC432 peptide

• Handling and Storage of Synthetic Peptides
• Material Safety Data Sheets (MSDS)

Batch to batch variation of the purity

XPKRRRRRR-NH2, X is 4-hydroxyphenylacetyl

9

95.5%

C₅₅H₁₀₀N₂₈O₁₀

1313.59

Synthetic

Powder

DC432 is a cell permeable analogue of BM30, also available, the first potent peptidomimetic substrate competitive inhibitor of N-terminal methyltransferase1/2 (NTMT1/2), one of a family of enzymes which methylate the α-N-terminal amines of proteins starting with the canonical X-P-K/R motif. DC432 has been made cell permeable by the addition of five arginine residues to BM30. DC432 decreases the N-terminal methylation level of the regulator of chromosome condensation 1 and SET proteins in HCT116 cells.

Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual: Handling and Storage of Synthetic Peptides

• Mackie et al (2020) Selective Peptidomimetic Inhibitors of NTMT1/2: Rational Design, Synthesis, Characterization, and Crystallographic Studies. J Med Chem. 63(17) 9512 doi:10.1021/acs.jmedchem.0c00689

Trifluoroacetic acid (TFA) is a common counterion from the purification process using High-Performance Liquid Chromatography (HPLC). The presence of TFA can affect the peptide's net weight, appearance, and solubility.

Impact on Net Weight: The TFA salt contributes to the total mass of the product. In most cases, the peptide content constitutes >80% of the total weight, with TFA accounting for the remainder.

Solubility: TFA salts generally enhance the solubility of peptides in aqueous solutions.

In Biological Assays: For most standard in vitro assays, the residual TFA levels do not cause interference. However, for highly sensitive cellular or biochemical studies, please be aware of its presence.